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Recombinant Protein Expression

We at Bon Opus Biosciences proudly offer industry-trusted and affordable recombinant protein expression services. Our distinct services offer the time and space to collaborate, fulfill, and exceed client requests in a reliable delivery window of 8 to 10 weeks. Our team of experienced and trusted scientists and researchers work diligently in ensuring each request receives the respect it deserves, offering our clients an affordable expression service with transparency, efficiency, and affordability. We carefully crafted our unique workflow to optimize insect expression with virus preparation to ensure accuracy and timely delivery. For custom protein expressions with insect cells, Bon Opus is your premier solution.  

 

Insect Recombinant Protein Expression Workflow 

 

Our general workflow follows a transparent step-by-step process, ensuring you’re comfortable with production and aware of potential challenges or favorable outcomes. We start by taking the constructed donor plasmid and transforming this into E. coli. E. coli is an integral part of protein expression services and requests. The E. coli to extract recombinant bacmid to begin transfecting insect cells. The additional step of virus preparation and generation contributes to the 8-to-10-week delivery window, but it is a vital step in the general workflow.  

From virus generation, we complete a virus expression process and enter purification and scaling. Upon completion of our insect protein expression system, we verify and review all details and pertinent data as it relates to your request. From there, we prepare your order for delivery. Our innovative and collaborative workflow sets the bar high for all available protein expressions, and we curated a system designed to fulfill our clients’ requests. 
 
We value the need for reliable recombinant protein expression services, and we work hard to keep our services affordable and accurate. Regardless of your request and origin, we can partner with you to define and deliver the needed insect or other host cell custom protein requests. We work with E. coli, yeast, mammalian, bacterial, and insect hosts, offering a wide range of custom services and solutions. Contact Bon Opus today to get started, or fill out our online contact form. We look forward to partnering with you! 

General Workflow

1. Construction of Recombinant Donor Plasmid

2. Transformation into E. coli

3. Extraction of Recombinant Bacmid 
& Transfection into Insect Cells

6. Scale-up & Purification

Virus titer plaque assay

Target protein

5. Virus Titration & Expression

Recombinant virus particles

4. Virus Generation

Steps Breakdown

Steps

Timeline

1) Gene synthesis, codon optimization, and DNA prep

2 weeks

2) Bacmid preparation ,transfection and expression evaluation

2-3 weeks

3) Infection of insect cells and titration of the virus

1-2 weeks

4) Expression, evaluation, and optimization

2 weeks

5) Scale-up Production

Inquiry

6) Final QC and Delivery of Product

Case Studies

Optimized Baculovirus Expression

  • Strategy: 

    • P1 Virus generation (transfection of insect cell with recombinant Bacmid DNA)

    • Expression test of P1 stock, WB test

    • 200mL P2 virus generation

    • 3 column purification using gel filtration/Ni affinity and ion-exchange column chromatography

  • Deliverables: 

    • 1.3 mg protein

    • >90% purity

P1 Transfection and P2 Virus Generation Data

Chromatography and Final Sample QC Data

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