Gene Synthesis Services
Bon Opus Biosciences offers reliable and affordable gene synthesis. We embrace a simple and transparent pricing structure, with a flat rate price of $0.23/bp for all fragments less than 3kb. We guarantee 100% sequence accuracy. Our on-time delivery (8-10 business days to U.S. locations) rate is over 95%.
Deliverables:
•5-10ug of lyophilized DNA in the standard vector (pUC57) Inquiry for all available vectors
•Certificate of analysis
•Sequence chromatogram
Available downstream services:
•Subcloning into custom vectors (customer provides vectors)
•DNA preparation (research grade, transfection grade, pre-clinical grade)
•Mutagenesis (single site and multiple sites)
Gene Fragments
Our gene fragment pricing is industry-low, with prices starting at just $75. See the table below for pricing, per fragment, based on fragment length.
DNA Prep
Our transfection and preclinical grade DNA prep options are both offered at extremely low endotoxin concentrations. See the table below for pricing; scroll down to see all research and pre-clinical grade options.
Gene Synthesis Process
Sequence optimization
Oligo synthesis
Gene assemble
Cloning
QC
The provided DNA sequence is analyzed by evaluating the GC content and repetitive sequences, and optimizing the codon sequence according to the protein expression system required. (Completed with the Quote)
To design the oligos required for gene synthesis, oligo analysis software is used to determine how to divide the gene into shorter fragments and design the oligos. The length of the oligos is usually around 100bp. Subsequently, all the oligos are synthesized in one oligonucleotide synthesizer, which is completed by the end of Night 1.
The synthesized oligos are assembled into longer fragments, typically 6-700bp, through PCR and PCR assembly. For genes less than 1.5kb, such as mAb seq, the complete gene can be obtained at this step. The fragments are then cloned into PUC57 and sequenced through fragment sequencing to verify their accuracy. For genes more than 1.5kb, a few more rounds of PCR will be used to amplify the entire gene.
The synthesized gene is cloned into a plasmid vector by either enzyme digestion or seamless cloning (Gibson).
Sanger sequencing is performed to ensure the correct sequence of the synthesized gene and enzyme digestion to verify the size of resulting DNA fragments. We guarantee 100% accuracy of the synthetic DNA sequences.
To submit your gene synthesis or gene fragment inquiry, simply fill out the form below:
WHAT PEOPLE SAY
ApexImmune Therapeutics
Bon Opus has provided us with excellent gene synthesis and protein expression and purification services. Their design team is always accommodating to our requests and highly detailed in their project planning. Their deliverables are also spot on. We look to Bon Opus for all our gene synthesis and protein reagent production needs.
Dr. Qian Zhang,
Scripps Research Institute
The Bon Opus team is truly efficient, experienced and reliable for gene synthesis and cloning. They have successfully helped us synthesize the VH and VL genes from a monoclonal antibody and then clone them into their antibody expression vectors. Their scientists were very attentive and responsive. They even noticed one of the aa sequences I submitted was missing two residues in the very N-terminus so that we could fix the mistake in time. The final products were delivered on time, of great quality, and at very competitive pricing. Overall, we are very satisfied with their service and will continue using their gene synthesis products.
Dr. Ying Wang,
Sorrento Therapeutics
[Bon Opus] provided us with high-quality gene fragment services, even when the sequence had high GC content or a strong secondary structure. There were no mutations involved during the sequencing of our cloned gene in the plasmids. The QC data for their gene synthesis services is exceptionally clear and helpful. I have not yet worked with another company whose data proved to be so valuable. [Bon Opus]’ service is very good overall and I would highly recommend to anyone.
Bon Opus Biosciences is the leading gene synthesis service provider with a reliable team of expert scientists and researchers elevating our processes. We pride ourselves on our ability to offer affordable and transparent services to each customer we serve, giving us (and our products) an edge over the competition. We deliver our core values and missions through each of our distinct services to help maintain a partnership built on integrity, flexibility, quality, and a proactive approach. When you need superior molecular biology services, Bon Opus is the solution for you.
Our reliable and comprehensive gene synthesis solutions offer a wide range of benefits, such as guaranteed accuracy, on-time delivery, and fair pricing. We offer a dynamic and transparent approach to gene fragment and DNA preparation, offering you a firsthand look at our price per quantity, DNA prep grades, and gene fragment lengths. Every member of our team treats your inquiry with the time and space it deserves to receive a comprehensive and accurate delivery that meets or exceeds your expectations.
Our ability to impress our customers through our gene and DNA synthesis services makes us the go-to supplier for gene fragments, DNA prep, custom protein productions, , recombinant antibodies, and much more. We accommodate a variety of requests and needs throughout our partnerships and offer a collaborative, solution-oriented approach from the initial contact point through our gene synthesis service’s completion. We strive for operational and knowledgeable excellence, and it shows in our provided services and project completion portfolio.
The Latin phrase bonum opus translates to “good work,” and with Bon Opus Biosciences leading your molecular biology services, good work is exactly what you will receive. We carefully crafted a transparent, collaborative, innovative method for each unique partnership we take on. Our experienced and education-rich team of scientists and researchers is ready to work with your distinct needs. Fill out our gene fragment or DNA prep inquiry form to get started, or connect with a member of our reputable team. Bon Opus Biosciences is the solution and service your sciences deserve.