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Gene Synthesis Services

Bon Opus Biosciences offers reliable and affordable gene synthesis. We embrace a simple and transparent pricing structure, with a flat rate price of $0.19/bp for all fragments less than 3kb and free-of-charge cloning to standard vector. We guarantee 100% sequence accuracy. Our on-time delivery (8-10 business days to U.S. locations) rate is over 95%.

Deliverables:

•5-10ug of lyophilized DNA in the standard vector (pUC57) Inquiry for all available vectors

•Certificate of analysis

•Sequence chromatogram

Available downstream services:​

•Subcloning into custom vectors (customer provides vectors) Free onboarding and storage

•DNA preparation (research grade, transfection grade, pre-clinical grade)

•Mutagenesis (single site and multiple sites)

DNA Preparation Price Table

*New Price!

Our transfection and preclinical grade DNA prep options are offered at extremely low endotoxin concentrations and take no longer than 5 days. See the table below for pricing; scroll down to see all research and pre-clinical grade options.

New Year
Better Prices

Gene synthesis starts at $0.19 per bp

1mg maxi prep starts at $99

Site-directed mutagenesis starts at $79

Gene Synthesis Process
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Sequence optimization

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Oligo synthesis

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Gene assemble

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Cloning

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QC

The provided DNA sequence is analyzed by evaluating the GC content and repetitive sequences, and optimizing the codon sequence according to the protein expression system required. (Completed with the Quote)

To design the oligos required for gene synthesis, oligo analysis software is used to determine how to divide the gene into shorter fragments and design the oligos. The length of the oligos is usually around 100bp. Subsequently, all the oligos are synthesized in one oligonucleotide synthesizer, which is completed by the end of Night 1.

The synthesized oligos are assembled into longer fragments, typically 6-700bp, through PCR and PCR assembly. For genes less than 1.5kb, such as mAb seq, the complete gene can be obtained at this step. The fragments are then cloned into PUC57 and sequenced through fragment sequencing to verify their accuracy. For genes more than 1.5kb, a few more rounds of PCR will be used to amplify the entire gene.

The synthesized gene is cloned into a plasmid vector by either enzyme digestion or seamless cloning (Gibson).

Sanger sequencing is performed to ensure the correct sequence of the synthesized gene and enzyme digestion to verify the size of resulting DNA fragments. We guarantee 100% accuracy of the synthetic DNA sequences.

WHAT PEOPLE SAY

ApexImmune Therapeutics

Bon Opus has provided us with excellent gene synthesis and protein expression and purification services. Their design team is always accommodating to our requests and highly detailed in their project planning. Their deliverables are also spot on. We look to Bon Opus for all our gene synthesis and protein reagent production needs.

Dr. Qian Zhang,
Scripps Research Institute

The Bon Opus team is truly efficient, experienced and reliable for gene synthesis and cloning. They have successfully helped us synthesize the VH and VL genes from a monoclonal antibody and then clone them into their antibody expression vectors. Their scientists were very attentive and responsive. They even noticed one of the aa sequences I submitted was missing two residues in the very N-terminus so that we could fix the mistake in time. The final products were delivered on time, of great quality, and at very competitive pricing. Overall, we are very satisfied with their service and will continue using their gene synthesis products.

Dr. Ying Wang,
Sorrento Therapeutics

[Bon Opus] provided us with high-quality gene fragment services, even when the sequence had high GC content or a strong secondary structure. There were no mutations involved during the sequencing of our cloned gene in the plasmids. The QC data for their gene synthesis services is exceptionally clear and helpful. I have not yet worked with another company whose data proved to be so valuable. [Bon Opus]’ service is very good overall and I would highly recommend to anyone.

Bon Opus Biosciences is an industry-leading expert in gene synthesis services and custom protein and antibody productions. Our in-lab team works closely with each customer to define and refine your distinct needs to develop high-throughput yields and comprehensive solutions that serve your needs best. We understand the importance of these requests being accurate and timely, and by taking you along with us for each step, this ensures your satisfaction. When it’s time to receive your molecular biology services, look no further than Bon Opus! 

The Primary Objective of Gene Synthesis 
During gene synthesis, or DNA synthesis services, the primary objective is crafting a distinct DNA sequence in the laboratory setting. Here at Bon Opus, we can craft and synthesize a number of genes and DNA sequences to serve a wide range of applications, and we cater our workflow to remain transparent and affordable to ensure those in need of services have access. Because of our distinct workflow, we can guarantee a 100 percent satisfaction rate, including accuracy and on-time delivery. We feature a comprehensive list of gene fragments with an industry low price starting at $75 per sequence. We also feature a robust inventory of preclinical DNA prep options with clinically low endotoxin concentrations, feasible for custom and experimental solutions. 

Choose Bon Opus Biosciences 
Our satisfaction guarantee and industry-leading affordability makes us the premier service provider for gene cloning services and much more. Our complete gene synthesis process uses a distinct five-step workflow sequence that includes sequence optimization, synthesizing Oligo, gene assembly, gene cloning, and a comprehensive quality control process. In each step, we take you with us to ensure you’re not only satisfied with the status, but ready to move forward to the next phase. We remain transparent in our approach to ensure any flaws in the synthesizing are handled with diligence as they serve your application best. 

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