TotumPro™
Custom Protein Production
Our protein team is led by a group of scientists with decades of experience in recombinant expression and relevant process development. The team has successfully produced over 1000 proteins per year in the past, and they are more than happy to take on a variety of recombinant expression projects, ranging from trial expression of rare protein variants, to gram-level production of antibodies and enzymes.
The overall success rate of our custom protein production projects is as high as 92%.
Features:
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Bundle with gene synthesis and cloning work
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Comprehensive chromatography selection
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Tag removal or no tag purification
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Systematic methods in solubility improvement
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Refolding (for E. coli expression)
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Gram-scale large capacity
Expression System:
-Our team of experts can provide advice on which system is suitable for your protein of interest.
Bacteria
Pros:
Scalable, low cost
Cons:
Solubility, lack of post-translational modifications (PTMs)
Mammalian
Pros:
Highest PTM, can express large and complex proteins
Cons:
High cost, demanding culture condition
Yeast
Pros:
Eukaryotic PTM, rapid growth
Cons:
Growth condition requires optimization
Insect
Pros:
Similar to mammalian PTM, high yield
Cons:
Slow growth rate, demanding culture condition
Popular Protein Production Packages
Order Workflow:
Customer Inquiry via online form or email
PM confirms the specifics and send quotes
Project starts once purchase orders received
Delivery
Citations
Service Cited: Custom Protein Production (E. coli)
Su, L. el al. CAR-T Engager Proteins Optimize Anti-CD19 CAR-T Cell Therapies for Lymphoma, bioRxiv,2022
Service Cited: Custom Protein Production (Mammalian cells)
Service Cited: Custom Protein Production (Mammalian cells)
Service Cited: Custom Protein Production (Mammalian cells)
Zhou, C. et al. An IL-17F.S65L Knock-In Mouse Reveals Similarities and Differences in IL-17F Function in Oral Candidiasis: A New Tool to Understand IL-17F. The Journal of Immunology, 2020 June 29
Service Cited: Custom Protein Production (Mammalian cells)
WHAT PEOPLE SAY
Hadassah Medical Center for Cancer Immunotherapy
We have a long and fruitful working relationship with the Bon Opus team. We have a tremendous appreciation for their valuable scientific support and excellent recombinant protein production. We received several batches of our custom-made protein with very good reproducibility. We also want to compliment the team's technical and scientific support that was always available. We will definitely continue working with Bon Opus in the future.
Dr. Luis Gimenez-Lirola,
Iowa State University
My long-term relationship with Bon Opus Biosciences is based on high quality work, flexibility, quick turnaround times, and outstanding personal customer service. Aside from excelling in customized projects, they offer a broad portfolio of products at extremely competitive prices. They are always available for questions and open to discuss alternatives for custom projects that are technically complicated. I will definitively continue working with them as they have become a crucial part of my research workflow.
Aleta Biotherapeutics
Aleta Biotherapeutics has a very good working relationship with Bon Opus. We have obtained more than 5 batches of protein produced by Bon Opus, including His tagged recombinant proteins and antibody-based proteins ranging from a 5 mg to 50 mg scale. The proteins produced by Bon Opus were of high quality with very low percentage aggregation and low endotoxin level. The representative we worked with at Bon Opus was very receptive to what we required for the product and delivered it to our satisfaction. We are happy to have Bon Opus as a reliable partner and will continue the collaboration with Bon Opus for future projects.
Dr. Harbani Malik,
TeneoBio
Bon Opus Biosciences has been able to provide us several reagents for our research projects. They are easy to work with and provide excellent communication updates throughout the process each time. When technical difficulties/problems arose during the project, the team was able to quickly provide several great suggestions for different strategies regarding protein production. [Bon Opus] also has highly competitive pricing.