Recombinant Antibody ServiCEs

One-stop Solution of Recombinant Antibody

Why Choose Bon Opus Biosciences For Custom Recombinant Antibody Production?

Our team possesses extensive expertise in the field of recombinant antibody production. We proudly provide a comprehensive solution that encompasses a wide range of antibody fragment formats.

Our services span from initial hybridoma sequencing and gene synthesis to the intricate stages of transient/stable cell line expression, meticulous purification and QC, and thorough characterization. This one-stop solution can be tailored to your specific needs, ensuring a seamless and efficient process from start to finish.

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Antibody Production Service Package

Altum mAb ™ HTP services

Comprehensive Modalities: mAb, VHH, ScFv, Fabs, BiSpecifics
Various Purification Tags: Fc, His, FLAG or no Tag
Large Capacity: >1500 per Month
Flexible Scales: 1ml, 4ml and 10ml
Choice of Deliverable: Expression Supernatant, Purified Antibodies
Optional Bioanalytical Assay: SPR and BLI
Proved Success Rate: >90% over 30,000 mAbs

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Pilot Scale Production Package from $645 per mAb:

Gene to Antibodies
From mg to Gram
High Purity: 98% by SEC-HPLC
Low Endotoxin: <0.1EU/mg

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Large Scale mAb Expression and Purification

Gene to Gram scales up
Fast Transient Transfection
Cell Line Pool Services

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Biochemical Reports

IgG

Mouse IgG2a

Rat IgG2a

Rabbit IgG

Human IgG1

IgA

Monomeric form

mhIgA1 (160kDa)

2 constructs

Heavy chain
Light chain

Dimeric form

dhIgA1 (320kDa)

3 constructs

Heavy chain
Light chain
J chain

Secretory form

shIgA1 (390kDa)

4 constructs

Heavy chain
Light chain
J chain
Secretory component

IgM

Pentameric form

human IgM (900kDa)

3 constructs

Heavy chain
Light chain
J chain

100mg Large Scale mAb Expression and Purification

Purification by MabSelect SuRe LX and Superdex SEC

MabSelect SuRe LX

Superdex 200

M. Molecular weight marker

FT: Flow through

W: Washes

E: Elution

Final Sample

SDS-PAGE

SEC-HPLC

Deliverable: 100mg purified antibody with 98% SEC-HPLC purity, endotoxin<0.1EU/mg
Turnaround time: 3 weeks

Antibody-antigen Affinity Analysis

At Bon Opus, we offer label-free, real-time biomolecular interaction analysis using both BLI (Bio-Layer Interferometry ) and SPR (Surface Plasmon Resonance)—powerful technologies for studying protein binding, affinity, kinetics, and quantitation.

SPR

SPR uses polarized light to excite surface plasmons on a metal surface. When target molecules bind, they shift the resonance angle of reflected light, revealing precise real-time data on binding kinetics and affinity.

Ideal for: Low-abundance targets, sensitive kinetic profiling, and small molecule interactions

BLI

BLI analyzes the interference pattern of white light reflected from a biosensor tip. As molecules bind or dissociate from an immobilized protein layer, the optical thickness changes—causing a measurable shift.

Ideal for: High-throughput screening, crude samples, and routine binding assays

Services Provided:

Affinity Measurement/kinetic
- Provides precise affinity measurements using SPR and BLI to determine how strongly two molecules bind—delivering key data such as association/dissociation rates and equilibrium constants (Kd) for proteins, antibodies.
Affinity Ranking
- Enables high-throughput ranking of candidate molecules based on binding kinetics and affinity, helping you identify the best binders quickly and confidently during lead optimization or screening campaigns.

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