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Recombinant Antibody ServiCEs

Antibody Production Service Package

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  1. Gene synthesis and codon optimization of VH and VL regions

  2. Cloning into premade Fc vectors*

  3. Transfection-grade plasmid preparation

  4. Transient expression in 20ml Expi293 or ExpiCHO

  5. One step Protein A or Protein L purification

  6. QC analysis: SDS-PAGE

Deliverable:

- Purified antibodies (>500ug in most cases), >95% purity (SDS-PAGE)

- 5ug mini prep of expression vectors

Turnaround time:

3-4 weeks plus shipping

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*Available Fc including Human IgG1, IgG2, IgG4, IgA1, Human Kappa, Lambda light chain, Mouse IgG1, IgG2a, Mouse Kappa light chain, Rat IgG2B, Rat Kappa light chain, etc.

$950 Pilot Scale mAb Production Package

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-Up to gram level mAb production with customized QC standard.

  1. Gene synthesis and codon optimization of VH and VL regions

  2. Cloning into premade Fc vectors

  3. Transfection-grade plasmid preparation

  4. Transient expression in Expi293 or ExpiCHO

  5. Purification by MabSelect SuRe LX and additional column upon request

  6. Endotoxin removal as low as 0.1EU/mg (optional) 

  7. QC analysis: SDS-PAGE, SEC-HPLC, endotoxin level test

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Deliverable:

- Purified antibodies with customized QC standard

Turnaround time:

4 weeks plus shipping

Large Scale mAb Expression and Purification
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