Recombinant Antibody Production ServiCE
Recombinant antibodies are antibody fragments generated in vitro through the use of synthetic genes. In contrast to the traditional antibodies produced by hybridoma technology, recombinant antibodies offer numerous advantages, including the preservation of high standards of specificity and low levels of immunogenicity.
Our team possesses extensive expertise in the field of recombinant antibody production. We proudly provide a comprehensive solution that encompasses a wide range of antibody fragment formats.
Our services span from initial hybridoma sequencing and gene synthesis to the intricate stages of transient/stable cell line expression, meticulous purification and QC, and thorough characterization. This one-stop solution can be tailored to your specific needs, ensuring a seamless and efficient process from start to finish.
Antibody Production Service Package
$950 Pilot Scale mAb Production Package
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Gene synthesis and codon optimization of VH and VL regions
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Cloning into premade Fc vectors*
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Transfection-grade plasmid preparation
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Transient expression in 20ml Expi293 or ExpiCHO
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One step Protein A or Protein L purification
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QC analysis: SDS-PAGE
Deliverable:
- Purified antibodies (>500ug in most cases), >95% purity (SDS-PAGE)
- 5ug mini prep of expression vectors
Turnaround time:
3-4 weeks plus shipping
*Available Fc including Human IgG1, IgG2, IgG4, IgA1, Human Kappa, Lambda light chain, Mouse IgG1, IgG2a, Mouse Kappa light chain, Rat IgG2B, Rat Kappa light chain, etc.
Large Scale mAb Expression and Purification
-Up to gram level mAb production with customized QC standard.
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Gene synthesis and codon optimization of VH and VL regions
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Cloning into premade Fc vectors
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Transfection-grade plasmid preparation
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Transient expression in Expi293 or ExpiCHO
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Purification by MabSelect SuRe LX and additional column upon request
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Endotoxin removal as low as 0.1EU/mg (optional)
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QC analysis: SDS-PAGE, SEC-HPLC, endotoxin level test
Deliverable:
- Purified antibodies with customized QC standard
Turnaround time:
4 weeks plus shipping
Case Study
IgG
Mouse IgG2a
Rat IgG2a
Rabbit IgG
Human IgG1
IgA
Monomeric form
mhIgA1 (160kDa)
2 constructs
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Heavy chain
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Light chain
Dimeric form
dhIgA1 (320kDa)
3 constructs
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Heavy chain
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Light chain
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J chain
Secretory form
shIgA1 (390kDa)
4 constructs
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Heavy chain
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Light chain
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J chain
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Secretory component
IgM
Pentameric form
human IgM (900kDa)
3 constructs
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Heavy chain
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Light chain
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J chain
100mg Large Scale mAb Expression and Purification
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Purification by MabSelect SuRe LX and Superdex SEC
MabSelect SuRe LX
Superdex 200
M. Molecular weight marker
FT: Flow through
W: Washes
E: Elution
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Final Sample
SDS-PAGE
SEC-HPLC
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Deliverable: 100mg purified antibody with 98% SEC-HPLC purity, endotoxin<0.1EU/mg
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Turnaround time: 3 weeks
WHAT PEOPLE SAY
Dr. Chun Chen, Pliant Therapeutics
We have received recombinant antibody production services from [Bon Opus] and were extremely satisfied with the quality and the turnaround time. We will definitely continue to use their services more in the future.
Bon Opus Biosciences offers reputable recombinant antibody production services that researchers around the globe use and trust. We have a one-of-a-kind team of in-house scientists and researchers who have committed decades’ worth of time and energy to their craft. They understand the art of bioscience and biotechnology and create custom experiences for each of our partnerships. We pride ourselves on our delivery and commitment to our core values and mission. Our fundamental principles were built on transparency and a desire to create a space for scientific collaboration with all our partnerships. We uphold a commitment to affordability, proactive and proven approaches, and superior quality in production. Together, these values allow us to establish well-rounded and reliable recombinant antibody expression through yeast, mammalian, or bacterial variants.
Our team has in-depth experience in specialized mAb production, allowing Bon Opus to offer the most advanced and innovative approach throughout our processes. Throughout production, we ensure the partnership remains at the top of the priority ladder. This effort fosters a scientific collaboration experience in which you can follow along with the process. You are there with Bon Opus for each step in the process, including synthesis, cloning and transformation, plasmid preparations, expression development, purifications and removals, and our comprehensive quality control analysis approach. We offer a transparent approach to our production processes on expressions, reagents, and all other variations. We value our commitment to your standards and thoroughly review and test each deliverable prior to considering delivery methods. To learn more about our comprehensive approach to recombinant antibody production and the variants available, please fill out our simple online contact form. A trusted member of our Bon Opus Biosciences team will communicate with you in a timely manner.