Recombinant Antibody Production
recombinant
antibody Production

Recombinant antibodies are antibody fragments generated in vitro through the use of synthetic genes. In contrast to traditional antibodies produced by hybridoma technology, recombinant antibodies have many advantages, such as maintaining high standards of specificity and low immunogenicity levels. 

Our team has extensive expertise in the field of recombinant antibody production. We offer a comprehensive range of services as below.


-Pilot scale mAb

-Large scale mAb

-ScFv

-Fab

-Bispecific mAb

-Altumab High Throughput Service

Antobody Production Service Package

recombinant antibody production monoclonal antibody production antibody services
 

$950 Pilot Scale mAb Production Package

  1. Gene synthesis and codon optimization of VH and VL regions

  2. Cloning into premade Fc vectors*

  3. Transfection-grade plasmid preparation

  4. Transient expression in 20ml Expi293 or ExpiCHO

  5. One step Protein A or Protein L purification

  6. QC analysis: SDS-PAGE

Deliverable:

- Purified antibodies (>500ug in most cases), >95% purity (SDS-PAGE)

- 5ug mini prep of expression vectors

Turnaround time:

3-4 weeks plus shipping

*Available Fc including Human IgG1, IgG2, IgG4, IgA1, Human Kappa, Lambda light chain, Mouse IgG1, IgG2a, Mouse Kappa light chain, Rat IgG2B, Rat Kappa light chain, etc.

Large Scale mAb Expression and Purification

-Up to gram level mAb production with customized QC standard.

  1. Gene synthesis and codon optimization of VH and VL regions

  2. Cloning into premade Fc vectors

  3. Transfection-grade plasmid preparation

  4. Transient expression in Expi293 or ExpiCHO

  5. Purification by MabSelect SuRe LX and additional column upon request

  6. Endotoxin removal as low as 0.1EU/mg (optional) 

  7. QC analysis: SDS-PAGE, SEC-HPLC, endotoxin level test

Deliverable:

- Purified antibodies with customized QC standard

Turnaround time:

4 weeks plus shipping

 
 

Case Study

20mL Pilot Scale mAb Expression and Purification

  • One step purification by MabSelect SuRe LX

  M. Molecular weight marker

  1. Reducing sample

  2. Non-reducing sample

 

100mg Large Scale mAb Expression and Purification​

  • Purification by MabSelect SuRe LX and Superdex SEC

MabSelect SuRe LX

M. Molecular weight marker

FT: Flow through

W: Washes

E: Elution

Superdex 200

  • Final Sample

SDS-PAGE

SEC-HPLC

  • Deliverable: 100mg purified antibody with 98% SEC-HPLC purity, endotoxin<0.1EU/mg

  • Turnaround time: 3 weeks

WHAT PEOPLE SAY

Dr. Chun Chen, Pliant Therapeutics

We have received recombinant antibody production services from [Bon Opus] and were extremely satisfied with the quality and the turnaround time. We will definitely continue to use their services more in the future.