Recombinant Expression in Yeast Cells
Yeast cells are similar to mammalian cells, as they confer eukaryotic processing, folding, and post-translational modification. At the same time, a yeast expression system delivers high level expression. Bon Opus has established a very efficient system for yeast cell-based recombinant expression. It includes signal sequences, expression vectors, and a set of yeast strains (X33, GS115, KM71, and SMD1168). Our team incorporates the use of an antibiotic gradient to screen for transformants with the highest numbers of inserts. This ensures a high expression level in the follow-up production phase. The final product should be delivered within a total of 12 weeks. A typical work flow for yeast-based recombinant protein production is described below.