Recombinant Protein Expression in Yeast Cells
Similar to mammalian cells, yeast cells confer eukaryotic processing, folding, and post-translational modification. At the same time, a yeast expression system delivers high level expression. Bon Opus has established an efficient system for yeast cell recombinant protein expression, which includes the design of signal sequences, expression vectors, and a set of yeast strains (X33, GS115, KM71, and SMD1168). Our team incorporates the use of antibiotic gradients to screen for transformants with the highest numbers of inserts, and thus ensure a high expression level in the follow-up production phase.
Feature Package: TotumPro™ Yeast Protein Production ($2500 per 0.5mg)
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Gene synthesis and cloning into yeast expression vector
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Preliminary expression detection in yeast cells: 10 positive clones, SDS analysis, WB
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200ml fermentation & one-step purification (His) of optimal clone in yeast cells
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90% purity, tag-on protein, no endotoxin control
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6-8 weeks delivery time
General Workflow
1. Gene synthesis
2. Cloning & Transformation
3. Clone Selection & Expression
4. Purification
Steps Breakdown
Steps
Timeline
1) Gene synthesis, codon optimization, and DNA prep
2 weeks
2) Transformation into P. pastoris cells
1 week
3) Screening for transformants with multiple copies of inserts
1 week
4) Expression, evaluation, and optimization
2 weeks
5) Scale-up production
Inquiry
6) Final QC and delivery of product
