Protein Case Study: Mammalian Cell Expression System

Featured below is a protein production case study utilizing a mammalian cell expression system. For more details regarding our custom protein production services, click here. If you would like to browse the workflow utilized by our team in our protein production process in mammalian cells, click here.

  • Strategy: 

    • 2L Expi 293 cells culture

    • Purification using Ni-NTA column and gel filtration

  • Deliverables: 

    • 10 mg

    • Purity >99% by SEC-HPLC

    • Endotoxin < 1EU/mg

Pictured below is the SEC-HPLC test demonstrating a purity level of 99%:

The SEC-HPLC analysis was conducted through the utilization of Superdex 200, in which proteins are purified according to their size.

Pictured below is the SDS-PAGE analysis, demonstrating a high level of protein purity:

Extracellular Domain His tag Protein:

Mammalian cell expression system

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