AltumMab™
High Throughput Recombinant Antibody Production
Recombinant antibodies are antibody fragments generated in vitro through the use of synthetic genes. In contrast to traditional antibodies produced by hybridoma technology, recombinant antibodies have many advantages, such as maintaining high standards of specificity and low immunogenicity levels.
Service Description
In response to the fast-growing antibody screening demands, Bon Opus Biosciences offers a high throughput antibody production service. The proprietary AltumMab™ high throughput antibody production service enables the expression of hundreds of recombinant antibodies at once, from gene synthesis to the delivery of purified antibodies in 96-well plates. This service provides a cost-effective solution for researchers to screen therapeutic antibody drug candidates or reagent antibodies in a short time.
Applications:
This high throughput service aims to help scientists conduct pilot studies on a group of
antibody candidates and identify the interested targets more efficiently. Its applications
include but not limited to:
-
Epitope mapping
-
Ligand competition binding studies
-
Drug screening assays
-
Biophysical characterization
Workflow:
Gene Synthesis
Gene synthesis and codon optimization of VH and VL regions provided by customers
* Minimum 10 mAb to start
Cloning
Cloning sequences into selected Fc region vectors and scale up for transfection
Expression
High throughput transient transfection and expression in 3mL Expi293 or ExpiCHO
Purification
High throughput purification with Protein A magnetic beads and final QC * ELISA or Biacore affinity test is available with an extra fee
AltumMab™ Servece Package Details: start from $375 per pair of antibody!
Expression Package A
Step
1
2
Deliverables
Working Day
7
5-7
Service Type
Gene synthesis (variable region, VH/VL), subcloning into respective constant vectors
Transfection-grade plasmid prep, 3ml HTP transfection (Expi293/CHO)
Plasmid, Clone strain, Antibody Medium, SDS-PAGE data
Expression Package B
Step
1
2
3
Deliverables
Working Day
7
5-7
2-3
Service Type
Gene synthesis (variable region, VH/VL), subcloning into respective constant vectors
Transfection-grade plasmid prep, 3ml HTP transfection (Expi293/CHO)
Biacore T200 test of expression medium antibody concentration
Plasmid, Clone strain, Antibody Medium, SDS-PAGE, Biacore T200 concentration report
Expression Package C
Step
1
2
3
Deliverables
Working Day
7
5-7
3
Service Type
Gene synthesis (variable region, VH/VL), subcloning into respective constant vectors
Transfection-grade plasmid prep, 3ml HTP transfection (Expi293/CHO)
H-T purification (magnetic Protein A beads)
Plasmid, Clone strain, Purified antibody, SDS-PAGE, Nano-drop one data
Gene Package
Step
1
2
Deliverables
Working Day
7
3-5
Service Type
Gene synthesis (variable region, VH/VL), subcloning into respective constant vectors
Transfection-grade plasmid prep
Up to 100ug transfection-grade DNA, Sequencing report