AltumMab
High Throughput Recombinant Antibody Production

Service Description

In response to the fast-growing antibody screening demands, Bon Opus Biosciences offers a high throughput antibody production service. The proprietary AltumMab™ high throughput antibody production service enables the expression of hundreds of recombinant antibodies at once, from gene synthesis to the delivery of purified antibodies in 96-well plates. This service provides a cost-effective solution for researchers to screen therapeutic antibody drug candidates or reagent antibodies in a short time.

Applications

This high throughput service aims to help scientists conduct pilot studies on a group of

antibody candidates and identify the interested targets more efficiently. Its applications

include but not limited to:

  • Epitope mapping

  • Ligand competition binding studies

  • Drug screening assays

  • Biophysical characterization
     

Workflow


 

Gene Synthesis

 

Gene synthesis and codon optimization of VH and VL regions provided by customers

* Minimum 10 mAb to start
 

Cloning

 

Cloning sequences into selected Fc region vectors and scale up for transfection

Expression

 

High throughput transient transfection and expression in 3mL Expi293 or ExpiCHO

Purification

 

High throughput purification with Protein A magnetic beads and final QC * ELISA or Biacore affinity test is available with an extra fee

 

AltumMab Servece Package Details:  start from $375 per pair of antibody!

Expression Package A

Step

1

2

Deliverables

Working Day

7

5-7

Service Type

Gene synthesis (variable region, VH/VL), subcloning into respective constant vectors

Transfection-grade plasmid prep, 3ml HTP transfection (Expi293/CHO)

Plasmid, Clone strain, Antibody Medium, SDS-PAGE data

Expression Package B

Step

1

2

3

Deliverables

Working Day

7

5-7

2-3

Service Type

Gene synthesis (variable region, VH/VL), subcloning into respective constant vectors

Transfection-grade plasmid prep, 3ml HTP transfection (Expi293/CHO)

Biacore T200 test of expression medium antibody concentration

Plasmid, Clone strain, Antibody Medium, SDS-PAGE, Biacore T200 concentration report

Expression Package C

Step

1

2

3

Deliverables

Working Day

7

5-7

3

Service Type

Gene synthesis (variable region, VH/VL), subcloning into respective constant vectors

Transfection-grade plasmid prep, 3ml HTP  transfection (Expi293/CHO)

H-T purification (magnetic Protein A beads)

Plasmid, Clone strain, Purified antibody, SDS-PAGE, Nano-drop one data

Gene Package

Step

1

2

Deliverables

Working Day

7

3-5

Service Type

Gene synthesis (variable region, VH/VL), subcloning into respective constant vectors

Transfection-grade plasmid prep

Up to 100ug transfection-grade DNA, Sequencing report