Recombinant antibody production is an essential part of immune system function, in partnership with B cells. These processes rely on viable cellular technologies and proper handling, but sometimes, accidents happen. We’ll explore the four most common mistakes in antibody production and how to avoid them.
Improper Culture Conditions
One of the critical factors that can affect the production of recombinant antibodies is the culture conditions used to grow the host cells. If the culture conditions are not optimized, this can lead to poor growth of host cells, misfolded antibodies, reduced viability of host cells, and increased likelihood of contamination by other microorganisms. To optimize the culture conditions, factors such as the type of host cell, medium composition, pH, temperature, oxygenation, and agitation must be carefully controlled.
By carefully controlling these factors, it is possible to achieve high yields of properly folded recombinant antibodies with high biological activity.
Poor Vector Selection
The vector is the DNA molecule that transfers the amplified gene into the host cell. The vector must be chosen carefully to ensure it can accommodate the gene of interest and provide the necessary elements for its expression. Selecting the wrong vector can result in low expression levels, poor stability, or low purity of the final product.
Purification is a crucial step in the production of recombinant antibodies, as it ensures the final product is free of contaminants and has the desired purity. Inadequate purification can lead to low purity levels, decreased stability, or loss of activity of the final product.
A Lack of Communication
Partnering with a development lab with poor communication capabilities can also impact the antibody production experience and process. Communication is a vital asset for proper production because the transparency of the process relies on the partnership having an open-door policy. Additionally, effective communication about the desired results can assist the lab workers’ ability to produce ideal timelines and yields.
For example, a client might expect a six-to-eight-week turnaround on a purified sample. If the developing lab cannot secrete the antibodies, they must communicate with the client quickly to adjust the design and production processes for the antibody of interest.
Bon Opus Biosciences specializes in high throughput antibody screenings with full production transparency and collaborative communication. We can help you avoid these common mistakes in antibody production, and our team of professionals will work diligently to ensure your custom requests are properly produced. Partner with us today to learn more about our services.