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Hybridoma Sequencing

Overview

Accurate antibody characterization starts with reliable sequencing. Our specialized Variable Regions Sequencing service for hybridomas focuses on identifying the V(D)J regions of the heavy and light chain immunoglobulin transcripts. These regions determine the antigen-binding specificity and are essential for recombinant antibody development, engineering, and patent applications.

For more complete insight, our Full-Length Sequencing captures the entire transcript, including both variable and constant regions. This comprehensive approach ensures accurate isotype identification and enables seamless cloning and expression of fully functional recombinant antibodies.

Whether you're preserving valuable hybridoma clones or preparing for downstream expression and humanization, our sequencing services offer precise, publication-ready data to support your antibody research and development goals.

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Process:

Variable Regions Sequencing

Step 1: mRNA Extraction

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Step 2: RT-PCR, Identification, and Sequencing

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Step 3: Annotation of VR(D)J regions of the heavy and light Ig gene transcripts from IgG producing hybridoma

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Turnaround Time: 1 week

Full Length Sequencing

Step 1: mRNA Extraction

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Step 2: RT-PCR, Amplification, and Sequencing

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Step 3: Annotation of the full length heavy and light chain immunoglobulin gene transcripts

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Turnaround Time: 2 weeks

  1. A comprehensive project report

  2. GenBank formatted annotated sequences of the identified regions

  3. The corresponding sequencing trace files.

Deliverables: 


1. Healthy and fresh cell, cell number > 1x10e6 / vail, 2 Vails
2. Delivery with Dry ice

Sample Requirements:

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