A minipool cell line development is a collection of cells derived from a single transfection event and then divided into numerous small populations (mini-pools) in 96-well plates. This approach allows for the efficient screening of a large number of cells to identify high-producing clones before single-cell cloning, accelerating the overall cell line development process.

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The mini-pool approach offers several advantages:

• Faster identification of high-producing clones by screening a larger number of mini-pools, the chances of finding a high producer are increased. 

• Reduced time to reach early production material: Mini-pools can be used to generate material for toxicology studies and early clinical trials, potentially shortening the overall development timeline. 

• Early assessment of product quality: Analyzing the product produced by mini-pools can provide insights into the quality attributes of the final product.

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At Bon Opus, we provide expert guidance and reliable services to help you develop high-quality, productive, and stable cell lines tailored to your research or bioproduction needs.

• Average 10 folds increase compared to transient transfection

• Quick turnaround time: 2 months from gene synthesis to final delivered minipool cell line

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