Protein Case Studies: E. coli cell expression system

Listed below are several protein production case studies utilizing an E. coli expression system. For more details regarding our custom protein production services, click here. If you would like to browse the workflow utilized by our team in our E. coli protein production process, click here.

  • Protein requirement:

    • Protein needs to be soluble, bioactive, tag-free with no extra amino acid, 98% purity

  • Strategy: 

    • pSUMO design: increase protein solubility. Tag cleavage by SUMO protease

    • Shuffle E. coli strain: promote correctly disulfide bonded protein 

    • Ni affinity chromatography

    • Sumo digestion and IEX

    • SEC-HPLC test

  • Turnaround time: 5-6 weeks

  • Deliverables: 

    • 132 mg tag-free protein in lyophilized or liquid form, concentration >2.5mg/ml

    • Purity >98%

    • Endotoxin <50EU/mg

Pictured below is the SDS-PAGE analysis:

SEC-HPLC Test Demonstrates 98% purity:

Active, soluble and tag-free protein production:

E. coli cell expression system

  • Protein requirements:

    • 50mg

    • Endotoxin level < 1EU/ug

    • Reducing sample SDS-PAGE 95% purity

  • Strategy: 

    • His-tagged protein and tag-free protein

    • Dimer formation and purification by Ni-NTA column 

    • SDS-PAGE 

  • Deliverables: 

    • 50 mg S100A8/S100A9 heterodimer 

    • Purity >95% by reducing SDA-PAGE

    • Endotoxin < 1EU/ug

Pictured below is the SDS-PAGE analysis:

Human Heterodimer:

E. coli cell expression system

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