Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (N-His)
Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein is produced by our E.coli expression system and the target gene encoding Met1-Ala419 is expressed with a 6His tag at the N-terminus. Bon Opus Cat.# DRA31
PurityGreater than 95% as determined by reducing SDS-PAGE.
BackgroundCoronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
PackagingDry ice/ice packs
ALTnames2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
Immobilized SARS-CoV-2 (2019-nCoV) NP Antibody (6G9)(Cat#DA027) at 5μg/ml (100 μl/well) showing the binding to SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein-His (Cat#DRA31).
The ED50 of SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein-His (Cat#DRA31) is 68 ng/ml.
Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl,300 mM NaCl, 1mM EDTA,pH 8.0.