Recombinant Human Protein N-terminal Glutamine Amidohydrolase/NTAQ1
Recombinant Human NTAQ1 is produced by our E.coli expression system and the target gene encoding Met1-Cys205 is expressed with a GST tag at the N-terminus. Bon Opus Cat. #CK09
BackgroundHuman protein N-terminal glutamine amidohydrolase (WDYHV1) is an enzyme that in humans is encoded by the WDYHV1 gene, belongs to the NTAQ1 family.WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, which is an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. However,it does not act on substrates with internal or C-terminal glutamine andnon-glutamine residues in any position. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
PackagingDry ice/Polar packs
FormulationSupplied as a 0.2 μm filtered solution of PBS,100mM GSH,1% TritonX-100,15% glycerol,pH7.4.
ALTnamesProtein N-terminal glutamine amidohydrolase,WDYHV1,Protein NH2-terminal glutamine deamidase,N-terminal Gln amidase,Nt(Q)-amidase,C8orf32, NTAQ1, REF: C1009