The protein was lyophilized from a concentrated (0.5 mg/ml) solution of PBS.
Less than 0.05 ng/µg (0.5 EU/µg) of hFGF-2.
>98.0% as determined by Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 8., and Analysis by reducing and non-reducing SDS-PAGE gel
The activity was determined by the dose-dependant proliferation of mouse BALB/c 3T3 cells.
Reconstitution and Solubility
It is recommended to reconstitute the lyophilized hFGF-2 in water, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar. Dissolve gently; never vortex.
Lyophilized hFGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution and filter sterilization hFGF-2 should be aliquoted and frozen. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Amino Acid Sequence
The sequence of the first six N-terminal amino acids was determined and was found to be MPALPE
Dimers and Aggregates
Less than 10% dimers as determined by gel filtration chromatography and no aggregates.
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein sequences.
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. Human FGF2 is purified by proprietary chromatographic techniques. Cat# BP07013