Recombinant Human β-1,3-Glucuronyltransferase 3/B3GAT3 (C-6His)
Recombinant Human B3GAT3 is produced by our E.coli expression system and the target gene encoding Glu72-Val335 is expressed with a 6His tag at the C-terminus. Bon Opus Cat. #CK05
BackgroundHuman Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) is an enzyme that in humans is encoded by the B3GAT3 gene, belongs to the glycosyltransferase 43 family. B3GAT3 is involved in a number of biological processes such as catalyzing the formation of the glycosaminoglycan-protein linkage by way of a glucuronyl transfer reaction in the final step of the biosynthesis of the linkage region of proteoglycans, forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate, gGlycosaminoglycans biosynthesis, transfering a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans.It also plays a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins , hows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc and stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.
PackagingDry ice/Polar packs
FormulationSupplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaC,2mM EDTA,20% Glycerol,pH 8.0.
ALTnamesB3GAT3,Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3,Beta-1,3-glucuronyltransferase 3,Glucuronosyltransferase I,GlcAT-I,GlcUAT-I,Gal beta-1,3-Gal-R glucuronyltransferase,,, REF: C1005