Protein Case Study: Yeast Cell Expression System

Featured below is a protein production case study utilizing a yeast cell expression system. For more details regarding our custom protein production services, click here. If you would like to browse the workflow utilized by our team in our protein production process in yeast cells, click here.

  • Strategy:

    • ​200 mL yeast culture expression

    • Purification using Ni-NTA column

    • QC by SDS-PAGE and anti-His WB

  • Deliverables: 

    • 6.6 mg protein 

    • Purity >95% by reducing SDS-PAGE

Pictured below is the SDS-PAGE analysis:

Capture.PNG

Optimized Pichia pastoris Expression:

Yeast cell expression system