ZAP70 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of ZAP70 on human thymus tissue lysate using anti-ZAP70 antibody at 1/500 dilution.
Fig2: ICC staining ZAP70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining ZAP70 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesTyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase
BackgroundThe activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and e chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short 5 amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with z and undergoes tyrosine phosphorylation following TCR stimulation.(ET1706-42)