WNK1 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of WNK1 on A549 cell lysates using anti-WNK1 antibody at 1/1,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-WNK1 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-WNK1 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenSynthetic Peptide within Human WNK1 aa 100-200 (Cysteine residue).
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesSerine/threonine-protein kinase WNK1, Erythrocyte 65 kDa protein, Kinase deficient protein, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1
BackgroundSerine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.(ET7108-41)