Vimentin Mouse monoclonal Antibody IgG1

Vimentin Mouse monoclonal Antibody IgG1

SKU: BA110709-100µl

Fig1: Western blot analysis of Vimentin on different cell lysates using anti- Vimentin antibody at 1/2000 dilution.

Positive control: 

   Lane 1: A549 

   Lane 2: Hela 

   Lane 3: NIH/3T3 

   Lane 4: Mouse embryonic stem cell

Fig2: ICC staining vimentin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using vimentin antibody. Counter stained with hematoxylin.

Bon Opus Cat. #BA110709
  • Host Species; Species Reactivity

    Mouse; Human, Mouse, Rat
  • Immunogen

  • Application Summary

    WB, ICC, IHC, FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

  • Background

    Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. In essence, vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin has been shown to eliminate toxic proteins in JUNQ and IPOD inclusion bodies in asymmetric division of asymmetric division of mammalian cell lines. It has been used as a sarcoma tumor marker to identify mesenchyme. Methylation of the vimentin gene has been established as a biomarker of colon cancer and this is being utilized in the development of fecal tests for colon cancer. High levels of DNA methylation in the promotor region have also been associated with markedly decreased survival in hormone positive breast cancers.(EM0401)

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