VCP Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution.
Lane 1: L929
Lane 2: F9
Lane 3: A549
Lane 4: MCF-7
Lane 5: Hela
Lane 6: NIH/3T3
Lane 7: N2A
Lane 8: Jurkat
Lane 9: HepG2
Lane 10: A431
Lane 11: Mouse heart
Lane 12: Mouse brain
Lane 13: Mouse liver
Lane 14: Mouse kidney
Fig2: ICC staining VCP in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining VCP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Zebrafish
Application SummaryWB, ICC, IHC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesTransitional endoplasmic reticulum ATPase,15S Mg(2+)-ATPase p97 subunit,Valosin-containing protein
BackgroundValosin-containing protein (VCP) is a highly conserved, that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. VCP is involved in the formation of the transitional endoplasmic reticulum (tER). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome.(ER30603)