ULK1 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of ULK1 on different cell lysate using anti-ULK1 antibody at 1/1,000 dilution.
Lane 1: MCF-7
Lane 2: Mouse spleen
Lane 3: SH-SY5Y
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ULK1 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded rat skeleton muscle tissue using anti-ULK1 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesSerine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1
BackgroundSerine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.(ET1704-63)