UBE2C Mouse monoclonal Antibody IgG2a
Fig1: Western blot analysis of UBE2C on human UBE2C recombinant protein using anti-UBE2C antibody at 1/1,000 dilution.
Fig2: Western blot analysis of UBE2C on HEK293 (1) and UBE2C-hIgGFc transfected HEK293 (2) cell lysate using anti-UBE2C antibody at 1/1,000 dilution.
Fig3: Western blot analysis of UBE2C on Hela (1) and Raji (2) cell lysate using anti-UBE2C antibody at 1/1,000 dilution.
Host Species; Species ReactivityMouse; Human
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesKelch repeat and BTB domain-containing protein 8, T-cell activation kelch repeat protein
BackgroundThe ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target.(EM1706-48)