Topoisomerase II alpha Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of TOP2A on different cell lysates using anti-TOP2A antibody at 1/500 dilution.
Lane 1: Jurkat
Lane 2: NIH/3T3
Lane 3: A431
Lane 4: L929
Lane 5: A549
Lane 6: Human liver
Lane 7: HUVEC
Fig2: ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining TOP2A in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC, IHC, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesDNA topoisomerase 2-alpha,DNA topoisomerase II, alpha isozyme
BackgroundThis gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. It is essential during mitosis and meiosis for proper segregation of daughter chromosomes.(ER40124)