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©2020 by Bon Opus Biosciences, LLC.

TIMP-1 Mouse monoclonal Antibody IgG

SKU: BA113131-100µl
$279.00Price

Fig1: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (EM2001-03) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

Fig2: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (EM2001-03) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TIMP-1) Monoclonal Antibody, Unconjugated (EM2001-03) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

Bon Opus Cat. #BA113131
Size
  • Host Species; Species Reactivity

    Mouse; Human, Mouse, Rat
  • Immunogen

    Recombinant human TIMP-1
  • Application Summary

    WB, IHC-P, ICC/IF
  • Purification; Formulation

    affinity purified by Protein G .01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Background

    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.(EM2001-03)