SIRT3 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis on different lysates using anti-SIRT3 rabbit polyclonal antibody. Positive control:
Lane 1: A172
Lane 2: Mouse liver
Lane 3: NIH/3T3
Lane 4: Mouse kidney
Lane 5: F9
Fig2: Immunocytochemical staining of MCF-7 cells using anti-SIRT3 rabbit polyclonal antibody.
Fig3: Immunocytochemical staining of HepG2 cells using anti-SIRT3 rabbit polyclonal antibody.
Host Species; Species ReactivityRabbit; Human, Mouse
Application SummaryWB, ICC, IHC, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesNAD-dependent protein deacetylase sirtuin-3, mitochondrial,Regulatory protein SIR2 homolog 3,SIR2-like protein 3
BackgroundThe Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologues, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT3 is a NAD-dependent deacetylase that contains one deacetylase sirtuin-type domain. The SIRT3 protein is widely expressed and localizes to the mitochondira where it is processed by mitochondrial processing peptidase (MPP) to yield a final product. This processing is most-likely necessary for its enzymatic activity.(R1511-3)