SIRT3 Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of SIRT3 on human SIRT3 recombinant protein using anti-SIRT3 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of SIRT3 on HEK293 (1) and SIRT3-hIgGFc transfected HEK293 (2) cell lysate using anti-SIRT3 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of SIRT3 on PANC-1 cell lysate using anti-SIRT3 antibody at 1/1,000 dilution.
Host Species; Species ReactivityMouse; Human
Application SummaryWB, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesLysine-specific demethylase 2B, CXXC-type zinc finger protein 2, F-box and leucine-rich repeat protein 10 F-box protein FBL10, F-box/LRR-repeat protein 10, JmjC domain-containing histone demethylation protein 1B, Jumonji domain-containing EMSY-interactor methyltransferase motif protein, Protein-containing CXXC domain 2, [Histone-H3]-lysine-36 demethylase 1B
BackgroundThe Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologues, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT3 is a NAD-dependent deacetylase that contains one deacetylase sirtuin-type domain. The SIRT3 protein is widely expressed and localizes to the mitochondira where it is processed by mitochondrial processing peptidase (MPP) to yield a final product. This processing is most-likely necessary for its enzymatic activity.(EM1706-24)