SIRT1 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of SIRT1 on different cell lysates using anti- SIRT1 antibody at 1/1000 dilution.
Lane 1: Hela
Lane 2: F9
Lane 3: Jurkat
Fig2: Flow cytometric analysis of Hela cells with SIRT1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-SIRT1 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse
Application SummaryWB, IHC, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesNAD-dependent protein deacetylase sirtuin-1，Regulatory protein SIR2 homolog 1，SIR2-like protein 1
BackgroundSirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol.(ER130811)