SIRT1 Mouse monoclonal Antibody IgG2b
Fig1: ICC staining SIRT1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti- SIRT1 mouse mAb.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti- SIRT1 mouse mAb.
Host Species; Species ReactivityMouse; Human, Mouse
Application SummaryWB, ICC, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesNAD-dependent protein deacetylase sirtuin-1,Regulatory protein SIR2 homolog 1,SIR2-like protein 1
BackgroundSirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol.(M1506-3)