Sequestosome-1 Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of SQSTM1 on different cell lysates using anti-SQSTM1 antibody at 1/500 dilution.
Lane 1: Hela
Lane 2: HepG2
Fig2: ICC staining SQSTM1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityMouse; Human, Mouse, Rat
ImmunogenSynthetic Peptide (KLH-coupled) within human SQSTM1 40-100 aa.
Application SummaryWB, ICC, IHC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesEBI3-associated protein of 60 kDa
BackgroundSequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. It may play a role in titin/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. P62 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.(EM0704)