Restriction enzyme FokI (C-term) Rabbit polyclonal Antibody IgG

SKU: BA110586-100µl

Fig1: Western blot analysis on purified restriction enzyme FokI.



Bon Opus Cat. #BA110586
  • Host Species; Species Reactivity

    Rabbit; Planomicrobium okeanokoites
  • Immunogen

  • Application Summary

  • Purification; Formulation

    Immunogen affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Type-2 restriction enzyme FokI,Endonuclease FokI,Type II restriction enzyme FokI,Type IIS restriction enzyme FokI
  • Background

    The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.(R1501-3)

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