Renilla Luciferase Recombinant Rabbit monoclonal Antibody IgG

Renilla Luciferase Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111354-100µl
$279.00Price

Fig1: Western blot analysis of Renilla Luciferase on luciferase recombinant protein lysate using anti-Renilla Luciferase antibody at 1/1,000 dilution.

 

 

Bon Opus Cat. #BA111354
Size
  • Host Species; Species Reactivity

    Rabbit; Sea pansy
  • Immunogen

    Recombinant protein
  • Application Summary

    WB,ICC,IF
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Coelenterazine h 2-monooxygenase, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase
  • Background

    Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.(ET1602-45)

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