Recombinant SARS-CoV-2 (2019-nCoV) S1 Protein

Recombinant SARS-CoV-2 (2019-nCoV) S1 Protein

SKU: DRA35-50ug

Recombinant SARS-CoV-2 (2019-nCoV) S1 Protein is produced by our Mammalian expression system and the target gene encoding Gln14-Arg685 is expressed. Bon Opus Cat.# DRA35

  • Accession Number

  • Purity

    Greater than 85% as determined by reducing SDS-PAGE.
  • Background

    Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM etc.. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Expression

    Human Cells
  • Packaging

    Dry ice/ice packs
  • ALTnames

    S1 protein; 2019-nCoV S1 protein; coronavirus S1 Protein; cov S1 Protein
  • Bioactivity

    Immobilized 2019-nCoV S1 Protein (Cat#DRA35) at 2μg/ml (100 μl/well) can bind Human ACE-2-Fc(Cat#C05Y). The ED50 of Human ACE-2-Fc(Cat#C05Y) is 49 ng/ml .

    Immobilized Human ACE-2-Fc(Cat#C05Y) at 10μg/ml (100 μl/well) can bind 2019-nCoV S1 Protein* (Cat#DRA35).

    *: Biotinylated by NHS-biotin prior to testing.

    The ED50 of 2019-nCoV S1 Protein* (Cat#DRA35) is 1.18 ug/ml.

  • Formulation

    Supplied as a 0.2 μM filtered solution of PBS, pH 7.4.

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