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Ras Rabbit polyclonal Antibody IgG

SKU: BA110580-100µl
$279.00Price

Fig1: Western blot analysis of Ras on different cell lysates using anti-Ras antibody at 1/1000 dilution.

Positive control:   

Lane 1: PC12              

Lane 2: MCF-7 

Lane 3: 293T              

Lane 4: Mouse brain 

Lane 5: Mouse intestine   

Lane 6: Rat brain

Fig2: ICC staining Ras in HCT116 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining Ras in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA110580
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Peptide
  • Application Summary

    WB, ICC, IHC, FC
  • Purification; Formulation

    Peptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    GTPase (G,N,K)Ras
  • Background

    Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. This antibody is predicted to react with H-Ras, N-Ras and K-Ras.(ER40115)