RAN Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of RAN on human RAN recombinant protein using anti-RAN antibody at 1/1,000 dilution.
Fig2: Western blot analysis of RAN on HEK293 (1) and RAN-hIgGFc transfected HEK293 (2) cell lysate using anti-RAN antibody at 1/1,000 dilution.
Fig3: Western blot analysis of RAN on different cell lysate using anti-RAN antibody at 1/1,000 dilution.
Positive control: Line1: Hela Line2: NIH/3T3 Line3: A431 Line4: C6 Line5: Jurkat Line6: Hela Line7: COS7 Line8: Jurkat
Host Species; Species ReactivityMouse; Human, Mouse, Monkey, Rat
Application SummaryWB, IHC, ICC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesProbable ATP-dependent RNA helicase DDX20, Component of gems 3, DEAD box protein 20, DEAD box protein DP 103, Gemin-3
BackgroundThe small Ras-related protein Ran, also called TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). The abundant GEF, RCC1 (regulator of chromosome condensation 1), increases the rate at which Ran exchanges GDP for GTP. Ran GAP1 opposes the effects of RCC1 by increasing the rate at which Ran hydrolyzes GTP to GDP. A protein designated Ran BP1 has no intrinsic GAP activity, and functions as a GEF inhibitor deactivating RCC1 and thereby indirectly increasing the ratio of Ran GDP to Ran GTP. The Ran BP2 protein has been proposed as the Ran GTP docking site at the periphery of the nuclear pore complex.(EM1706-18)