RAD52 Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of RAD52 on human RAD52 recombinant protein using anti-RAD52 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of RAD52 on HEK293 (1) and RAD52-hIgGFc transfected HEK293 (2) cell lysate using anti-RAD52 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of RAD52 on different cell lysate using anti-RAD52 antibody at 1/1,000 dilution.
Positive control: Line1: HepG2 Line2: MCF-7 Line3: MCF-7 Line4: C6
Host Species; Species ReactivityMouse; Human
Application SummaryWB, ICC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesCalcium/calmodulin-dependent protein kinase type II subunit gamma
BackgroundRad52 family members (Rad50, Rad51B/C/D, Rad52, Rad54, MRE11) mediate DNA double-strand break repair (DSBR) for DNA damage that otherwise could cause cell death, mutation or neoplastic transformation. Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands. Rad52 can interact with Rad51. Rad54A of the DEAD-like helicase superfamily binds to double-strand DNA and induces a DNA topological change, which is thought to facilitate homologous DNA pairing, and stimulate DNA recombination. Rad54B of the DEAD-like helicase superfamily binds to double-stranded DNA and displays ATPase activity in the presence of DNA. Rad54B is abundant in testis and spleen, and mutations of this gene occur in primary lymphoma and colon cancer.(EM1706-14)