RACK1 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of RACK1 on different lysates using anti-RACK1 antibody at 1/2,000 dilution.
Lane 1: SH-SY-5Y
Lane 2: Rat small intestine
Fig2: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-RACK1 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-RACK1 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenRecombinant protein corresponding to C-terminal human RACK1.
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesReceptor of activated protein C kinase 1, Cell proliferation-inducing gene 21 protein, Guanine nucleotide-binding protein subunit beta-2-like 1, Guanine nucleotide-binding protein subunit beta-like protein 12.3, Human lung cancer oncogene 7 protein, Receptor for activated C kinase, Small ribosomal subunit protein RACK1, Receptor of activated protein C kinase 1, N-terminally processed, Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed
BackgroundMembers of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. Receptor for activated C kinases, termed RACKs, are intracellular receptors for activated PKC that may be involved in the activation-induced translocation of PKC. RACK1 (receptor for activated C kinase 1) is a 317 amino acid G protein b subunit-like protein that functions as a RACK and inhibits the activity of Src tyrosine kinases. In response to PKC activation, the intracellular localization of RACK1 and PKC bII changes, and RACK1 and PKC bII co-localize to the same sites. RACK1 is therefore thought to be a shuttling protein for PKC bII. A deficit in RACK1 may be associated with impaired PKC activation in the aging brain. The RACK1 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.pombe, S.cerevisiae, K.lactis, E.gossypii, M.grisea, N.crassa, A.thaliana, rice and P.falciparum, and maps to human chromosome 5q35.3.(ET7109-04)