RAB27A Mouse monoclonal Antibody IgG2b
Fig1: Western blot analysis of RAB27A on human RAB27A recombinant protein using anti-RAB27A antibody at 1/1,000 dilution.
Fig2: Western blot analysis of RAB27A on HEK293 (1) and RAB27A-hIgGFc transfected HEK293 (2) cell lysate using anti-RAB27A antibody at 1/1,000 dilution.
Fig3: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-RAB27A antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityMouse; Human
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesTestis development protein NYD-SP19
BackgroundThe Rab family of low molecular weight GTPases are critical regulators of vesicular transport. Rab proteins cycle between an active GTP-bound state, which recruits specific effector proteins, and an inactive GDP-bound state. Two members of this family, Rab27a and Rab27b, have overlapping functions, but differ in tissue specificity. Rab27a is widely expressed with significant expression in pancreatic islets and pituitary tissue, and low expression in brain. Rab27b is also expressed in pituitary tissue, but is more significantly expressed in brain and spleen. Rab27a regulates diverse processes involving lysosome-related organelles, including melanosome motility in melanocytes and lytic granule release in cytotoxic T lymphocytes. Mutations in the Rab27a gene result in Griscelli syndrome (GS) or the corresponding mouse model ashen, a rare autosomal recessive disorder characterized by hypopigmentation, prolonged bleeding times, and platelet storage pool deficiency (2,4). In GS, Rab27a is not available to mediate the recruitment of melanosomes via the actin motor, myosin Va. The human Rab27b gene maps to chromosome 18q21.1, and encodes a protein that is involved in pituitary hormone secretion. Rab27b may be functionally redundant to Rab27a, as it can rescue Rab27a mutants.(EM1706-32)