Pygopus 2 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis on NCCIT(1) and Hela(2) cell lysates using anti-Pygopus 2 rabbit polyclonal antibody.
Fig2: Flow cytometric analysis of MCF-7 cells with Pygopus 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Host Species; Species ReactivityRabbit; Human, Mouse
Application SummaryWB, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesPygopus homolog 2
BackgroundPygopus 2, also known as PYGO2, is a 406 amino acid protein that is the human homolog of the Drosophila pygopus protein. Localized to the nucleus, Pygopus 2 contains one PHD finger that interacts with the homology domain of the Wnt signaling protein Bcl-9. This interaction joins Pygopus 2 with the ∫-catenin/TCF complex (a crucial complex in Wnt signaling), thereby allowing ∫-catenin to transcriptionally activate Wnt target genes. Association of Pygopus 2 with proteins involved in the Wnt signaling pathway is thought to regulate proper signal transduction, as absence of the Pygopus 2/∫-catenin interaction may play a role in development of B-cell malignancies. In addition, Pygopus 2 expression is upregulated in and required for the growth of breast cancer cells, suggesting a crucial role in carcinogenesis.(R1510-27)