PTP1B Recombinant Rabbit monoclonal Antibody IgG
SKU: BA111994-100µl
$279.00Price
Fig1: Western blot analysis of PTP1B on different lysates using anti-PTP1B antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: MCF-7 Lane 3: HepG2 Lane 4: A431 Fig2: ICC staining PTP1B in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Fig3: ICC staining PTP1B in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Size
100 µl
Host Species; Species Reactivity
Rabbit; Human, ZebrafishImmunogen
Recombinant proteinApplication Summary
WB, ICC/IF, IHC, FCPurification; Formulation
ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.ALTnames
Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1BBackground
The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosine phosphatases. These latter enzymes bear little resemblance to either the protein serine and protein threonine phosphatases or to the acid and alkaline phosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein. On the basis of sequence analysis, PTP1B (PTPase 1B) expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, a homolog of the neural adhesion molecule (NCAM). PTP1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.(ET1701-90)