PRPF8 Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112581-100µl
$279.00Price

Fig1: Western blot analysis of PRPF8 on SiHa (1)  and A549 (2) cell lysate using anti-PRPF8 antibody at 1/1,000 dilution.

Fig2: ICC staining PRPF8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining PRPF8 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA112581
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Recombinant protein within 1–200 of human PRPF8.
  • Application Summary

    WB,ICC,IHC,FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Pre-mRNA-processing-splicing factor 8, 220 kDa U5 snRNP-specific protein, PRP8 homolog, Splicing factor Prp8, p220
  • Background

    PRP8, also designated pre-mRNA-processing-splicing factor 8, is a highly conserved nuclear protein and a central component of the catalytic core of the spliceosome, where it may be involved in various molecular rearrangements. PRP8, which is widely expressed, plays a role in transesterification reactions that regulate splicesome-induced pre-mRNA splicing. Specifically, PRP8 interacts with the GU dinucleotide at the 5' splice site (5'SS) and forms a specific UV-inducible cross-link. It also interacts functionally with the 3'SS, affecting the efficiency of the second catalytic step. PRP8 may play a role in the first transesterification step, as PRP8 mutations that prohibit negative regulation of PRP28 or PRP44/Brr2 subsequently block U4 activation. In addition, PRP8 interacts with a conserved region of U6 that is instrumental in the formation of the catalytic core of the spliceosome.(ET7108-18)

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