Proteasome 20S LMP2 Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112497-100µl
$279.00Price

Fig1: Western blot analysis of Proteasome 20S LMP2 on different lysates using anti-Proteasome 20S LMP2 antibody at 1/500 dilution.

 Positive control:

 Lane 1: Mouse spleen tissue 

      Lane 2: U937 

    Lane 2: Mouse colon tissue

 

Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Proteasome 20S LMP2 antibody. Counter stained with hematoxylin.

Fig3: Flow cytometric analysis of Daudi cells with Proteasome 20S LMP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Bon Opus Cat. #BA112497
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse
  • Immunogen

    Recombinant protein
  • Application Summary

    WB,IHC,FC,IP
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Proteasome subunit beta type-9, Low molecular mass protein 2, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Proteasome chain 7, Proteasome subunit beta-1i, Really interesting new gene 12 protein
  • Background

    The eukaryotic multicatalytic proteinase complex, otherwise known as the proteasome, is present in both the nucleus and cytoplasm of cells and contains at least 15 nonidentical subunits, which form a highly ordered ring-shaped structure. The proteasome is involved in an ATP/Ubiquitin-dependent proteolytic pathway and expresses at least five distinct proteolytic activities, including the cleavage of peptides after branched chain amino acids or bulky hydrophobic amino acids. Two components of the proteasome are the low molecular mass proteins LMP2 and LMP7, which are thought to connect the proteasome to the MHC class-I antigen-processing pathway. Upon stimulation with IFN-γ, LMP2 and LMP7 displace housekeeping subunits in the proteasome and activate cytotoxic T cells (CTLs). LMP2 and LMP7 are produced as precursor proteins, which are processed to subunits that have the ability to complex with the proteasome. LMP2 is expressed as two alternatively spliced forms, LMP2.l and LMP2.s, in lymphoblastoid cell lines and in fibroblasts after IFN-γ stimulation. LMP7 is also expressed as two forms, LMP7-E1 and E2, in several tissues.(ET7107-24)

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