PPP1CB Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of PPP1CB on human PPP1CB recombinant protein using anti-PPP1CB antibody at 1/1,000 dilution.
Fig2: Western blot analysis of PPP1CB on HEK293 (1) and PPP1CB-hIgGFc transfected HEK293 (2) cell lysate using anti-PPP1CB antibody at 1/1,000 dilution.
Fig3: Western blot analysis of PPP1CB on different cell lysate using anti-PPP1CB antibody at 1/1,000 dilution.
Positive control: Line1: Jurkat Line2: A431 Line3: Hela Line4: HepG2 Line5: HEK293 Line6: MCF-7
Host Species; Species ReactivityMouse; Human
Application SummaryWB, ICC, IHC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
BackgroundIn eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family. The PP1 family is comprised of subfamily members PP1α, PP1β and PP1γ, which are MgATP-dependent enzymes. PP1 inactivity is maintained through its association with the inhibitory protein NIPP-1 (nuclear inhibitor of PP1). Phosphorylation of NIPP-1 by cAMP-PK or casein kinase II results in the release of active PP1.(EM1706-97)