PICK1 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of PICK1 on SK-Br-3 cell lysate using anti-PICK1 antibody at 1/5,000 dilution.
Fig2: Flow cytometric analysis of 293T cells with PICK1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenRecombinant protein within N-terminal human PICK1.
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesPRKCA-binding protein, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein
BackgroundProtein interacting with C kinase 1 (PICK1) is a PDZ-domain containing protein that is located in the perinuclear region and is phosphorylated in response to PKCα activation. PKCα, which is essential for the regulation of proliferation and differentiation in numerous cell types, contains within its catalytic region a PDZ-binding domain that is absent from other PKC isoforms. Mutation of the PICK1 PDZ domain inhibits the binding of PICK1 to PKCα. PICK1 also interacts with the carboxy terminus of a-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor, a neurotransmitter receptor located at excitatory synapses, suggesting that PICK1 plays a role in the modulation of synaptic transmission by targeting and anchoring AMPA to specific synapses.(ET7108-60)