PI3-kinase p85 subunit alpha Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis on different cell lysates using anti-PI3-kinase p85 subunit alpha Mouse mAb. Positive control:
Lane 1: U-937
Lane 2: Raji
Fig2: Immunocytochemical staining of Hela cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.
Fig3: Immunocytochemical staining of MCF-7 cells using anti-PI3-kinase p85 subunit alpha Mouse mAb.
Host Species; Species ReactivityMouse; Human, Mouse, Rat
Application SummaryWB, ICC, IHC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesPhosphatidylinositol 3-kinase regulatory subunit alpha,Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
BackgroundPhosphatidylinositol 3-kinase (PI 3-kinase) is composed of (p85) and (p110) subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110δ expression is restricted to white blood cells. It has been shown to bind p85α and γ, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and βγ heterotrimeric G proteins.(M1510-2)