PI 3 Kinase p55 gamma Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of PI 3 Kinase p55 γ on different lysates using anti-PI 3 Kinase p55 γ antibody at 1/1,000 dilution.
Lane 1: MCF-7
Lane 2: Jurkat
Lane 3: Mouse testis
Fig2: ICC staining PI 3 Kinase p55 γ in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PI 3 Kinase p55 γ in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC/IF, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesPhosphatidylinositol 3-kinase regulatory subunit gamma, Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma, p55PIK
BackgroundPhosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3’ position. PI 3-kinase p55γ (PIK3R3) is comprised of a catalytic subunit and a regulatory subunit. The human p55γ protein is composed of a rare amino terminal region followed by a proline-rich motif and two Src homology 2 (SH2) domains. PI 3-kinase p55γ mRNAs are expressed in most human fetal and adult tissues; predominant expression is observed in the adult testis. Splice variant(s) of PI 3-kinase p55γ have been identified; one of which has a deletion of 36 amino acids at the amino terminus and another which has an insertion of 59 amino acids at position 256 between the SH2 domains. Research suggests that PI 3-kinase p55γ interacts with the IGFIR (Insulin-like growth factor-I receptor) and IR (Insulin receptor) and may be involved in PI 3-kinase activation by these receptors.(ET1602-27)