Phospho-Stat5 (Y694) Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Phospho-Stat5(Y694) on mouse liver lysates using anti-Phospho-Stat5(Y694) antibody at 1/1,000 dilution.
Host Species; Species ReactivityRabbit; Human, Mouse
ImmunogenSynthetic phospho-Peptide corresponding to residues surrounding Tyr694 of human Stat5.
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesSignal transducer and activator of transcription 5A, Signal transducer and activator of transcription 5B
BackgroundStat5 (Signal Transducers and Activators of Transcription 5) is important in regulating T cell functions involving the receptors for Interleukin-2 (IL-2). IL-2 stimulates the rapid phosphorylation of both serine and tyrosine residues of Stat5a and Stat5b in human T lymphocytes and in several IL-2-responsive lymphocytic cell lines. IL-2 differentially induces serine phosphorylation of Stat5a and Stat5b on Ser726 and Ser731, respectively. Stat5b is preferentially phosphorylated and displays more protracted serine phosphorylation kinetics than Stat5a. Both the acid-rich region and the COOH terminus of IL-2Rb can independently mediate IL-2-induced Stat 5a/b serine phosphorylation, suggesting that Stat5a/b serine phosphorylation occurs at a postreceptor level. Stat5a is phosphorylated on Tyr694 in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive. Activation of Stat5 by IL-2 may help govern the biological effects of IL-2 during the immune response.(ET1610-48)