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Phospho-Smad5 (S463/465) Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111423-100µl
$279.00Price

Fig1: Western blot analysis of Phospho-Smad5(S463/465) on different lysates using anti-Phospho-Smad5(S463/465) antibody at 1/1,000 dilution.

Positive control: 

  Lane 1: Mouse brain 

  Lane 2: Rat brain

Fig2: ICC staining Phospho-Smad5(S463/465) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining Phospho-Smad5(S463/465) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA111423
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Synthetic phospho-Peptide corresponding to residues surrounding Ser463 and 465 of human Smad5.
  • Application Summary

    WB, ICC, IHC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Mothers against decapentaplegic homolog 5, JV5-1, SMAD family member 5
  • Background

    Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.(ET1605-5)