Phospho-Smad2 (S250) Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Phospho-Smad2 (S250) on Hela cells lysates using anti-Phospho-Smad2 (S250) antibody at 1/1,000 dilution.
Fig2: Flow cytometric analysis of Hela cells with Phospho-Smad2 (S250) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenSynthetic phospho-Peptide corresponding to residues surrounding Ser250 of human Smad2.
Application SummaryWB, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesMothers against decapentaplegic homolog 2, JV18-1, Mad-related protein 2, SMAD family member 2
BackgroundSmad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.(ET1612-32)