Phospho-Rac1+Cdc42 (Ser71) Recombinant Rabbit monoclonal Antibody IgG
Fig1: ICC staining Phospho-Rac1+Cdc42(Ser71) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining Phospho-Rac1+Cdc42(Ser71) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Phospho-Rac1+Cdc42(Ser71) antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human
ImmunogenSynthetic phospho-Peptide corresponding to residues surrounding Ser71 of human CDC42.
Application SummaryWB, ICC/IF, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesCell division control protein 42 homolog, G25K GTP-binding protein, Ras-related C3 botulinum toxin substRate 1, Cell migRation-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1
BackgroundA large number of low molecular weight GTP-binding proteins of the Ras superfamily have been identified in eukaryotic cells; they regulate many fundamental processes such as cell growth, vesicle traffic and cytoskeletal organization. GTPase-activating proteins accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in downregulation of their active form. Rac 1 is activated in a type I interferon (IFN) dependent manner; its function is required for downstream engagement of the p38 MAP kinase pathway. The p38 MAP kinase plays an essential role in IFN-dependent transcriptional regulation. The serine/threonine kinase Akt, of the phospho-inositide 3-kinase signal transduction pathway phosphorylates serine 71 of Rac 1. The superfamily of GTP-binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of diverse biological activities involving various aspects of cell growth and division.One mammalian member of the family, Cdc42, has an amino acid sequence that is similar to those of various members of the Ras superfamily proteins, including N-, K- and H-Ras, Rho proteins and the Rac proteins. On the basis of in vitro phosphorylation studies, it has been suggested that human Cdc42 may function in the signaling pathway of the EGF receptor or related growth factor receptor protein kinases. The Dbl oncogene has been shown to specifically catalyze dissociation of GDP from human Cdc42.(ET1701-40)