Phospho-PKC zeta (T560) Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Phospho-PKC zeta(T560) on mouse eyeball lysates using anti-Phospho-PKC zeta(T560) antibody at 1/1,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-PKC zeta(T560) antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-PKC zeta(T560) antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenSynthetic phospho-Peptide corresponding to residues surrounding Thr560 of human PKC zeta.
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesProtein kinase C zeta type, nPKC-zeta
BackgroundMembers of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, Ω, h, θ, λ/ι, and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.(ET1611-78)